The new fee will cover the cost of matching up the driver's number plate with their tolling account.
Sydney’s Harbour Bridge and tunnel tolls will add an extra slug for vehicle matching.
Motorists using the Harbour Bridge or tunnel are set to be stung with a new charge each time they don't hear that familiar beep from their e-tag.
From June 1 motorists will be charged an extra 55c “vehicle matching” fee on top of the regular toll when they cross the Harbour if they don't travel with a tag or it fails to register.
Previously a photograph was taken of the car's number plate and the toll was automatically added to the account linked to that car at no extra cost.
A Roads and Maritime Services spokeswoman said the new fee would cover the cost of matching up the driver's number plate with their tolling account.